2021-07-07 Meeting notes

Date

Jul 7, 2021

Participants

  • @Jeffrey Wagner

  • @Connor Davel

  • @Owen Madin

Discussion topics

Item

Notes

Item

Notes

Molecules from Simon

  • CD – I’m unable to reproduce connectivity changes from Simon’s report. This is probably due to the code I sent him initially. When I try to run these using the new (rdkit-based) ways of finding connectivity changes, I don’t see the connectivity changes. I suspect that the root cause of the cases he found are due to OE automatically adding protons.

    • JW – If the cases here are due to an OE molecule interpretation error, then this probably isn’t relevant for our look into proton migration. Could you clarify what we’re looking to do with this step?

    • CD – We start with an sqm.in file containing coordinates but no bonds. Then we end up with a sqm.out file with coordinates but no bonds. Then we can use various AmberTools to turn that into a sdf or mol2 file. I’ve had trouble getting either of those into RDKit. So I tried using OpenEye to read them and that’s where I hit this problem.

    • JW – Could we move forward with this just using RDKit to guess the final connectivity?

    • CD – That’s looked OK in my experimentation, so I think that would be fine.

  • CD – I’d like to send a new script to Simon and have him re-run it.

    •  

    • CD – I’m a bit concerned overall because SB only found 9 cases in a set of about 50k.

    • (General) – We could incorporate protomer enumeration into the molecule-loading routine: protomers = mol.enumerate_protomers()

      • We’ll want to generate the protomers before we generate conformers

    • OM – Could get you set up on Summit eventually, or on a Shirts lab workstation sooner.

      • OM will get CD an account on the Shirts lab network

    • (General) – CD will work on testing the new connectivity rearrangement workflow on a Shirts lab workstation. Once that’s tested+running CD will send the updated script to SB, though SB will be on vacation for a week.

Trying out ways to remediate proton migrations

  • Let’s check whether SHAKE is really being applied to these systems, since many of the outputs looks like its not

  • We verified that the total charges on molecules are being calculated correctly from the input SDFs now that we have the carboxylate bond stuff fixed.

Action items

Decisions